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Size (kb): 5.6799998283386230

Vector: pSELECT-1 (phagemid)

Promoters: Promoter T7

Construction: pBR322, pGEM-3Zf(+)

Marker(s):tetR

Construct size (kb): 5.679999828338623

Features: insert detection: lacZ'
marker(s): tetR
promoter: lac, SP6, T7
replicon: pMB1, f1

vector containing primer sites useful for sequencing

vector permitting RNA synthesis in vitro

vector permitting visual detection of recombinants

vector useful for in-vitro mutagenesis

Restriction digests of the clone give the following sizes (kb): EcoRI--6.2; BamHI--6.2; HindIII--6.2.

This vector contains the following unique restriction sites: ClaI, EcoRV, StyI, ScaI and AatII.

Useful for in vitro mutagenesis experiments. Encodes the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.

Contains an inactive ampicillin resistance gene which can be reverted to functional expression by a repair oligonucleotide.

Temperature: 37.0°C