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PYS-2
PYS-2
規(guī)格:
貨期:
編號(hào):B212468
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 PYS-2
商品貨號(hào) B212468
Organism Mus musculus, mouse
Tissue yolk sac; parietal endoderm
Cell Type epithelial
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease carcinoma
Age embryo
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
osteonectin (BM-40)
Cellular Products
osteonectin (BM-40)
Comments
SPARC is an extracellular Ca+2 binding glycoprotein, which is differentially associated with morphogeneisis, remodeling, cellular migration and proliferation [PubMed; 2745554]. Exogenous SPARC, added to cells in culture is associated with profound changes in cell shape, caused rapid, partial detachment of a confluent monolayer, and inhibited spreading of newly plated cells [PubMed; 2745554].
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor EH Sage
Deposited As mouse
References

Sage H, et al. SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix. J. Cell Biol. 109: 341-356, 1989. PubMed: 2745554

Basu A, et al. The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO. Mol. Cell. Biol. 17: 677-686, 1997. PubMed: 9001221

Satyamoorthy K, et al. Identification of an intronic enhancer that nullifies upstream repression of SPARC gene expression. Nucleic Acids Res. 25: 3169-3174, 1997. PubMed: 9224620

Lehman JM, et al. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma. J. Cell. Physiol. : 13-27, 1974. PubMed: 4858519

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