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CHAROMID 9-20
CHAROMID 9-20
規(guī)格:
貨期:
編號(hào):B211608
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) CHAROMID 9-20
商品貨號(hào) B211608
Designations CHAROMID 9-20
Depositors I Saito
Biosafety Level 1
Vector Information
Size (kb): 20.0000000000000000
Vector: CHAROMID 9-20 (cosmid)
Construction: pTBE-PL9 and multiple copies of a 2 kb AvaI spacer
Marker(s):ampR
Construct size (kb): 20.0
Features: marker(s): ampR
replicon: pMB1
terminator: none
enhancer: none
Applications
Host should be mk+ because some packaging mixes destroy charomids by EcoK.
Useful for cloning 18-32 kb fragments.
Comments
Dimer vector can be packaged, so cosmid-type 'arm' preparation or phosphatase treatment is necessary. Host should be mk+ because some packaging mixes destroy charomids by EcoK.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Useful for cloning 18-32 kb fragments.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Saito I, Stark GR. Charomids: Cosmid vectors for efficient cloning and mapping of large or small restriction fragments. Proc. Natl. Acad. Sci. USA 83: 8664-8668, 1986. PubMed: 3022301

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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