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Human Coxsackievirus A6
Human Coxsackievirus A6
規(guī)格:
貨期:
編號(hào):B211548
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Human Coxsackievirus A6
商品貨號(hào) B211548
Classification Picornaviridae, Enterovirus, Human enterovirus A
Agent Human Coxsackievirus A6
Strain Gdula
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C
Images ATCC VR-1801 Photomicrographs
Comments Derived by adaptation of primary cell culture product ATCC® VR-165™ to RD cells (ATCC® CCL-136™).  ATCC® VR-165™ and ATCC® VR-1801 have not been compared for sequence of infectivity in primary cell culture and tissue culture.
Effect on Host
CPE, cell rounding and death
Recommended Host
RD cells (ATCC® CCL-136™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer with DPBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor HA Wenner
Source Stool from 5-year-old child with sore throat, fever and leg muscle spasm, New York, 1949.
References

Dalldorf G. The coxsackie virus group. Ann. N.Y. Acad. Sci. 56: 583-586, 1953. PubMed: 13139263

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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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