99精品久久99久久久久,亚洲国产成人精品无码一区二区,国产精品亚洲综合色区韩国,久久精品第一国产久精国产宅男66,亚洲国产日韩欧美一区二区三区,久久久久久精品免费无码无,国产中年熟女高潮大集合,亚洲AV无码成H在线观看

Distribution host: Escherichia coli JM109

Size (kb): 7.0000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 7.000
Type of vector: plasmid
Cloning sites: PstI HincII PvuI ScaI SmaI
Polylinker sites:
Construction: pDM2, pBGS8
Host range: Haemophilus influenzae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR
replicon: pMB1, pRSF0885

Vector: pVT66 (plasmid)

Construction: pDM2, pBGS8

Marker(s):ampR,cmlR

Construct size (kb): 7.0

Features: marker(s): ampR, cmlR
replicon: pMB1, pRSF0885

shuttle vector

Restriction digests of the clone give the following sizes (kb): PstI--7.0; PvuI--7.0; SmaI--7.0; BamHI--4.7, 2.4.

Cloning into the HincII, PstI, PvuI, or ScaI sites inactivates ampR.

Cloning into the SmaI site inactivates cmlR.

Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid.

Shuttle vector.

The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR.

Temperature: 37.0°C