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pVT66
pVT66
規(guī)格:
貨期:
編號:B211520
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pVT66
商品貨號 B211520
Designations pVT66
Depositors VN Trieu
Biosafety Level 1
Host
Distribution host: Escherichia coli JM109
Vector Information
Size (kb): 7.0000000000000000
DESCRIPTION OF VECTOR:
Intact vector size: 7.000
Type of vector: plasmid
Cloning sites: PstI HincII PvuI ScaI SmaI
Polylinker sites:
Construction: pDM2, pBGS8
Host range: Haemophilus influenzae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR
replicon: pMB1, pRSF0885
Vector: pVT66 (plasmid)
Construction: pDM2, pBGS8
Marker(s):ampR,cmlR
Construct size (kb): 7.0
Features: marker(s): ampR, cmlR
replicon: pMB1, pRSF0885
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): PstI--7.0; PvuI--7.0; SmaI--7.0; BamHI--4.7, 2.4.
Cloning into the HincII, PstI, PvuI, or ScaI sites inactivates ampR.
Cloning into the SmaI site inactivates cmlR.
Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid.
Shuttle vector.
The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Trieu VN, McCarthy D. Improved plasmid shuttle vectors for Haemophilus influenzae and Escherichia coli. Gene 86: 99-102, 1990. PubMed: 2179060

Vuong N Trieu, personal communication

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