99精品久久99久久久久,亚洲国产成人精品无码一区二区,国产精品亚洲综合色区韩国,久久精品第一国产久精国产宅男66,亚洲国产日韩欧美一区二区三区,久久久久久精品免费无码无,国产中年熟女高潮大集合,亚洲AV无码成H在线观看

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Naegleria morganensis Dobson et al.
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Naegleria morganensis Dobson et al.
Naegleria morganensis Dobson et al.
規(guī)格:
貨期:
編號(hào):B211496
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Naegleria morganensis Dobson et al.
商品貨號(hào) B211496
Deposited As Naegleria sp.
Strain Designations SWL NG-236
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Fresh water, River Murray, Morgan, Australia, 1984
Product Format frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain yes
Comments
Zymodeme 44-2
Equals NG-236, maximum temperature tolerance 44C
Medium ATCC® Medium 997: Fresh water ameba medium
ATCC® Medium 711: PYB
Growth Conditions
Temperature: 25°C
Cryopreservation Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Page's Balanced Salt Solution (or similar), 8.5 mL


Harvest and Preservation 
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL ATCC medium 1323 (Page's Balanced Salt Solution) and washing cells into suspension.  Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.  Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.   Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 997.  Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 25°C.
  10. Follow the protocol for maintenance of culture.


Name of Depositor BS Robinson
Year of Origin 1984
References

Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158

Dobson PJ, et al. New thermophilic Naegleria species (Heterolobosea: Vahlkampfiidae) from Australia and Asia: allozyme, morphometric and physiological characterization. Acta Protozool. 36: 261-271, 1997.

type strain

De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
西贡区| 武邑县| 莒南县| 遵义市| 大方县| 康马县| 乌审旗| 中牟县| 黄大仙区| 禹州市| 天水市| 逊克县| 南京市| 彩票| 海盐县| 巴东县| 蕉岭县| 蒲江县| 宜兰县| 农安县| 涟源市| 奇台县| 故城县| 潞城市| 新兴县| 烟台市| 襄樊市| 安康市| 龙江县| 博白县| 读书| 罗甸县| 连江县| 莱西市| 建宁县| 潮州市| 公主岭市| 涟源市| 崇信县| 驻马店市| 靖宇县|