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Human rhinovirus 15
Human rhinovirus 15
規(guī)格:
貨期:
編號:B211085
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Human rhinovirus 15
商品貨號 B211085
Classification Picornaviridae, Rhinovirus
Agent Human rhinovirus 15
Strain 1734
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This product is a mycoplasma free preparation derived from VR-285. It replaces ATCC VR-1125 and ATCC VR-285, which will be discontinued when inventory is depleted. Strain 1734 is the prototype strain for human rhinovirus 15 and it has been fully sequenced (GenBank: DQ473493).
Product Format frozen
Storage Conditions ?Store at -70°C or colder
Genome Sequenced Strain

Yes

Comments
This product is a mycoplasma free preparation derived from VR-285. It replaces ATCC VR-1125 and ATCC VR-285, which will be discontinued when inventory is depleted. Strain 1734 is the prototype strain for human rhinovirus 15 and it has been fully sequenced (GenBank: DQ473493).
Effect on Host
Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells.
Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells.
Growth Medium ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host:H1-HeLa (ATCC CRL-1958)

Production Host: ?H1-HeLa (ATCC? CRL-1958™)

Alternate Hosts:WI-38 (ATCC CCL-75 ), HeLa (ATCC CCL-2 ), KB (ATCC CCL-17 ) and other human cells.
Growth Conditions
For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired. Freeze preparation as rapidly as possible.
Name of Depositor KM Johnson, RM Conant, VV Hamparian
Source
Presumed from throat swab from young adult with upper respiratory illness, South Carolina, 1960; this preparation derived from ATCC VR-285 following treatment to remove mycoplasma.
References

Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967.

Kistler AL, et al. Genome-wide diversity and selective pressure in the human rhinovirus. Virol. J. 4: 40, 2007. PubMed: 17477878

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