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Leishmania donovani (Laveran and Mesnil) Ross
Leishmania donovani (Laveran and Mesnil) Ross
規(guī)格:
貨期:
編號:B211073
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Leishmania donovani (Laveran and Mesnil) Ross
商品貨號 B211073
Strain Designations Khartoum (kh)
Application
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
human, Khartoum, Sudan, 1943
Product Format frozen
Type Strain no
Comments
Amastigotes.
Medium ATCC® Medium 811: Leishmania medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Cryopreservation

1.?? Harvest cells from cultures that are at or near peak density. Aseptically transfer the broth overlay to a plastic centrifuge tube and adjust the concentration of cells to 2 x 107/ml in fresh medium. If necessary, cells may be concentrated by centrifugation at 800 x g for 5 min.

2.? Prepare a 10% (v/v) solution of sterile DMSO in fresh medium.? Cool on ice.

3.? Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO solution to the start of the freezing process should be no less than 15 min and no longer than 30 min.

4.? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

5.?? Place the vials in a controlled rate freezing unit.? From room temperature cool at -1°C/min to -40°C.? If the freezing unit can compensate for the heat of fusion, maintain rate at??????? -1°C/min through the heat of fusion.? At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately

????? -1°C/min.)?

6.? The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -70°C.

7.?? To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.

8.?? Do not leave in the water bath. Immediately after thawing aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube of fresh ATCC medium 807 or 811.

9.?? Incubate the culture vertically at 25°C. Observe the culture daily and transfer when numerous trophozoites are observed.

Name of Depositor R Herman
Chain of Custody
ATCC <<--R Herman<<--L. Stauber <<--- W. Meleney
Year of Origin 1943
References

Stauber LA. Characterization of strains of Leishmania donovani. Exp. Parasitol. 18: 1-11, 1966. PubMed: 5909670

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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