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pGB29
pGB29
規(guī)格:
貨期:
編號:B211048
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGB29
商品貨號 B211048
Designations pGB29
GenBank Number

J05411

Species Elizabethkingia miricola
Depositors Genzyme Corp., G Barsomian, Genzyme Corp.
Applications
produces protein peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F
Vector
Construct size (kb): 6.200
Insert
DNA: genomic
Insert lengths(kb): 3.30
Gene product: peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F [ngl]
Target Gene: peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F
Insert Size (kb) 3.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried E. coli containing the phagemid.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; Sau3AI--1.0, 0.7, 0.6, + smaller; BglII--3.5, 3.2.
The sequenced portion of the insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--1.33; HindIII--0.80; NdeI--0.85; PvuII--0.93; EcoRI--1.6. Additional EcoRI sites are known to occur beyond the site indicated.
Contains the complete coding sequence (nt 139-1203). A subclone deleting EcoRI fragments downstream of the gene was used for sequencing and expression studies.
Proteins of 35, 37 and 38 kDa are expressed. Expression is independent of orientation in the vector, suggesting the presence of an endogenous promoter that functions in E. coli.
The vector was constructed from pBluescript SK+ by converting ApaI and SacI sites into BglII sites.
The insert in this clone was isolated from ATCC 33958, Elizabethkingia miricola, formerly Flavobacterium meningosepticum.
References

Barsomian GD, et al. Cloning and expression of peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F in Escherichia coli. J. Biol. Chem. 265: 6967-6972, 1990. PubMed: 2182635

Barsomian GD, et al. Endo F-free PNGase. US Patent 5,238,821 dated Aug 24 1993

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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