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pKB663
pKB663
規(guī)格:
貨期:
編號(hào):B210857
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pKB663
商品貨號(hào) B210857
Designations pKB663
GenBank Number

M10431

Species Escherichia coli (Migula) Castellani and Chalmers
Depositors OmniGene, Inc., KC Backman, OmniGene, Inc.
Applications
produces protein chorismate mutase-P-prephenate dehydrogenase
Vector
Construct size (kb): 5.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pKB430
Intact vector size: 4.500
Type of vector: plasmid
Vector end: PvuII
Vector end: BamHI
Cloning sites: PvuII
Polylinker sites:
Other unique sites: BamHI
Construction: pBR322, lac promoter
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, ->
replicon: pMB1
promoter for expression: lac, ->
restriction site: PvuII
marker(s): tetR, <-
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: pheA
Gene name: chorismate mutase-P-prephenate dehydrogenase
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert 5' end: StuI
Insert 3' end: BglII
Insert size (kb): 2.17
Cross references: DNA Seq. Acc.: M10431
Nucleotides 1-2168 of the insert correspond to
nucleotides 576-2743 of M10431.
Insert lengths(kb): 2.170000076293945
Gene product: chorismate mutase-P-prephenate dehydrogenase [pheA]
Target Gene: chorismate mutase-P-prephenate dehydrogenase
Insert Size (kb) 2.170
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; EcoRI/BamHI--4.0, 1.08; EcoRI/NcoI--3.5, 1.5.
Excision of the insert with EcoRI+Tth111I yields a lac promoter - pheA cassette.
The construct contains a weak attenuator sequence at the 5' end of the pheA coding sequence.
Classification Enterobacteriaceae, Escherichia
References

Backman KC. Method of biosynthesis and cells therefor. US Patent 4,839,286 dated Jun 13 1989

Backman KC. Enzyme deregulation. US Patent 4,753,883 dated Jun 28 1988

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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