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Trypanoplasma borreli Laveran and Mesnil
Trypanoplasma borreli Laveran and Mesnil
規(guī)格:
貨期:
編號(hào):B210850
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Trypanoplasma borreli Laveran and Mesnil
商品貨號(hào) B210850
Strain Designations El
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
blood of pike, Esox lucius, Czech Republic
Product Format frozen
Type Strain no
Comments
blood parasite of fish
Medium ATCC® Medium 2213: Biphasic trypanoplasma medium (SNB-9)
Growth Conditions
Temperature: 20.0°C
Duration: axenic
Cryopreservation
1.?? Harvest cells from several cultures in the late logarithmic or early stationary phase of growth.? Vigorously agitate by inverting several times to suspend the cells.? Maintain cells on ice between manipulations.

2.?? Aseptically transfer the cell suspension to 15 ml plastic centrifuge tubes.

3.?? Centrifuge at ~800 x g for 5 min.

4.?? While cells are centrifuging, prepare a 10% solution of DMSO in liquid overlay from ATCC medium 2213.? Cool on ice.

5.?? Remove the supernatant and pool the cell pellets to the final volume desired with fresh medium overlay.

6.?? Combine the cell suspension with an equal volume of 10% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 5% DMSO.

7.?? Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).

8.?? Place the vials in a controlled rate freezing unit.? From room temperature cool at -1°C/min to -40°C.? At -40°C, plunge ampules into liquid nitrogen.? Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.). ?

9.?? Store ampules in a liquid nitrogen refrigerator until needed.

10.????????? To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.? Allow the ampule to thaw completely (2-3 min).

11.????????? Immediately after thawing, aseptically remove the contents and transfer to a fresh test tube of ATCC Medium 2213.

12.Screw the cap on tightly and incubate at 15-20°C on a 15° horizontal slant.? Observe the culture daily and transfer when numerous trophozoites are observed.?????????

Name of Depositor EJ Noga
Chain of Custody
ATCC <<--EJ Noga<<--J. Lom
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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