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lambdafoo
lambdafoo
規(guī)格:
貨期:
編號:B210690
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 lambdafoo
商品貨號 B210690
Designations lambdafoo
Depositors IN Maruyama
Biosafety Level 1
Vector Information
Size (kb): 43.0000000000000000
DESCRIPTION OF VECTOR:
Intact vector size: 43.000
Type of vector: phage
Cloning sites: HindIII BamHI SacI EcoRI
Polylinker sites: HindIII BamHI SacI EcoRI
Construction: lambda2001, lambda gene V
Host range: Escherichia coli
Features (with orientation and position when available):
coding sequence: gene V N-terminal 176 aa, ->
other: amber stop codon, ->
restriction site: SfiI
other: Pro-Thr box encoding a linker peptide, ->
insert detection: lacZ', ->
ribosome-binding site: Shine-Dalgarno, ->
initiation codon: ATG, ->
MCS: HindIII...EcoRI, ->
Vector: lambdafoo (phage, lambda - replacement)
Construction: lambda2001, lambda gene V
Construct size (kb): 43.0
Features: initiation codon: ATG
insert detection: lacZ'
other: Pro-Thr box encoding a linker peptide
other: amber stop codon
MCS: HindIII...EcoRI
restriction site: SfiI
ribosome-binding site: Shine-Dalgarno
coding sequence: gene V N-terminal 176 aa
Applications
expression vector
vector for constructing cDNA libraries
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--33.0, 9.4; EcoRI--33.0, 9.4; BglII--22.0, 8.8, 4.8, 4.6, 3.1; PstI--9.6, 9.0, 4.6, 3.2, 2.9, 2.8, 2.4, 2.2, 1.9, 1.9, 1.5.
Vector allowing expression of cloned inserts as a fusion protein on the phage particle surface. Inserts are fused to the C-terminus of a truncated phage tail protein by a peptide linker.
Presence of the lacZalpha coding sequence and ribosome binding site allow blue-white color detection of recombinants, as well as allowing expression of a cloned insert separate from the phage tail protein.
The Pro-Thr box encodes alternating prolyl and threonyl residues, which form a link between the N-terminal phage tail protein and the foreign protein.
The linker resembles the hinge-region of IgA1 and allows separation of the foreign protein from the phage particles by digestion with enzymes such as Cellulomonas fimi protease or collagenase.
Production of large amounts of fusion protein may inhibit phage assembly. A host allowing low efficiency suppression of the amber mutation is recommended.
Media ATCC® Medium 1592: SM buffer
Growth Conditions
Temperature: 37.0°C
References

Maruyama IN, et al. Lambda foo: a lambda phage vector for the expression of foreign proteins. Proc. Natl. Acad. Sci. USA 91: 8273-8277, 1994. PubMed: 8058794

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