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pCu425CTR1-CYC1
pCu425CTR1-CYC1
規(guī)格:
貨期:
編號:B210639
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCu425CTR1-CYC1
商品貨號 B210639
Designations pCu425CTR1-CYC1
Depositors S Labbe, DJ Thiele
Biosafety Level 1
Vector Information
Size (kb): 7.4609999656677250
DESCRIPTION OF VECTOR:
Intact vector size: 7.461
Type of vector: phagemid
Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI
Polylinker sites: SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI Xho
Construction: pRS425, CTR1-CYC1 promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, <-
replicon: f1, <-
terminator: CYC1, <-
MCS: XhoI...SpeI, ->
promoter for expression: CTR1-CYC1, ->
replicon: pMB1
marker(s): ampR, <-
replicon: 2 micron, <-
Vector: pCu425CTR1-CYC1 (phagemid)
Promoters: Promoter for expression CTR1-CYC1
Construction: pRS425, CTR1-CYC1 promoter
Marker(s):LEU2,ampR
Construct size (kb): 7.460999965667725
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CTR1-CYC1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
Applications
cloning vector
Comments
Restriction digests of the vector give the following sizes (kb): SacI--7.5; SacI/SpeI--7.0, 0.38.
CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activate under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid.
References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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Shipping Information Distributed: DNA supplied by depositor (frozen) (amount: 100 ng)
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