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Expression from this construct has been used to quantitate p53 expression levels.

The following primers can be used for RT-PCR to detect transcription of the cloned insert: Forward 5' - CATCTACAAGCAGTCACA - 3'; Reverse 5' - GCAGTGCTCGCTTAGTGC - 3'. The amplified product size is 442 bp.

The following oligonucleotide sequence can be used as an internal probe to detect the amplified product: 5' - ATCCACTACAACTACATG - 3'.

Construct size (kb): 3.299999952316284

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4Z
Intact vector size: 3.000
Type of vector: plasmid
Vector end: HincII
Vector end: HincII
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TP53
Genomic copy number: unique
Gene name: tumor protein p53 (Li-Fraumeni syndrome)
Contains complete coding sequence?: N
Chromosome: 17; Localization: 17 p13.1
Tissue: SV40-transformed fibroblast
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.6
Cross references: DNA Seq. Acc.: X02469
Nucleotides 1-567 of the insert correspond to
nucleotides 600-1167 of X02469.

Insert lengths(kb): 0.6000000238418579

Tissue: SV40-transformed fibroblast

Gene product: tumor protein p53 (Li-Fraumeni syndrome)( tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen) [TP53]

Alleles: D2, C2, C1, A1, A1, B2, B1, D1, A2, A2

Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--2.7, 0.6; EcoRI--3.3; BamHI--3.3.

Expression from this construct has been used to quantitate p53 expression levels.

The following primers can be used for RT-PCR to detect transcription of the cloned insert: Forward 5' - CATCTACAAGCAGTCACA - 3'; Reverse 5' - GCAGTGCTCGCTTAGTGC - 3'. The amplified product size is 442 bp.

The following oligonucleotide sequence can be used as an internal probe to detect the amplified product: 5' - ATCCACTACAACTACATG - 3'.

Insert was isolated from php53B plasmid DNA (ATCC 57255) and subcloned into a vector allowing RNA transcription.