Vector |
Construct size (kb): 4.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ', <- primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, <- promoter: lac, <- primer site: M13 reverse, <- replicon: pMB1 marker(s): ampR, <- Cross references: |
Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ATP1A2 Genomic copy number: unique Gene name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide Contains complete coding sequence?: U Chromosome: 1; Localization: 1 q21-q23 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 0.7 Cross references: DNA Seq. Acc.: M78212; EST: EST01805; Primer: M13 Forward; THC id: THC122363 DNA Seq. Acc.: M86176; EST: EST02708; Primer: M13 Reverse; THC id: THC122363 Insert lengths(kb): 0.699999988079071 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Gene product: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide [ATP1A2] |
Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 0.7; BamHI--4.0; PvuII--2.8, 1.2. EST01805 matches GenBank accession HUMATP1A2 with 99.64% identity over the sequence range: 1-278. EST02708 contains an Alu repeat element. |
References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
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