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pZilch-NLS
pZilch-NLS
規(guī)格:
貨期:
編號(hào):B171773
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pZilch-NLS
商品貨號(hào) B171773
Designations pZilch-NLS
Depositors C Hovens
Biosafety Level 1
Vector Information
Size (kb): 6.1059999465942380
Vector: pZilch-NLS (phagemid)
Promoters: Promoter CMV
Construction: pCATCH-NLS
Marker(s):ampR
Construct size (kb): 6.105999946594238
Features: marker(s): ampR
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: BamHI...XhoI
MCS: BglII...XmaI
signal peptide coding region: SV40 NLS
terminator: SV40
coding sequence: mutant pheS gene
epitope tag: FLAG
Applications
encodes removable tag for protein isolation
expression vector
vector for assessing nuclear localization signals
vector permitting construction of fusion proteins
vector permitting positive selection for inserts
vector permitting production of single-stranded DNA
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1.
The vector was contructed by inserting a 1185 bp SmaI/BamHI fragment from pKSS encompassing the mutant pheS allele into the pCATCH-NLS and modified further to generate polylinker and to incorporate Kozak sequence and initiation ATG codon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Schneider S, et al. An epitope tagged mammalian/prokaryotic expression vector with positive selection of cloned inserts. Gene 197: 337-341, 1997. PubMed: 9332383

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梅經(jīng)理 17280875617 1438578920
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