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p114.12
p114.12
規(guī)格:
貨期:
編號:B171734
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p114.12
商品貨號 B171734
Designations p114.12
GenBank Number

X01179

Species Homo sapiens, human
Depositors RM Lawn
Vector
Construct size (kb): 3.0
Insert
DNA: genomic
Insert lengths(kb): 0.6470000147819519
Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) [F8C]
Alleles: F3, E1, E1, E2, F1, F2, A1, A2
Insert Size (kb) 0.647
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.5; EcoRI--3.4; PstI--3.0, 0.6; BamHI/SstI--2.8, 0.7.
Of 93 obligate carriers for hemophilia A, 35 were informative for the BclI RFLP (exon 17-18). Of 39 obligate carriers NOT detected by p114.12, 19 were informative with p482.6 (intron 22/XbaI).
Insert includes exons 17-18 extending slightly 3' to exon 18 but not as far as the polymorphic BclI site.
BclI polymorphism maps to intron 18.
Enzyme(s) not detecting polymorphism: AhaIII, ApaI, AvaII, BamHI, BamHII, BglI, BstEII, BstXI, EcoRI, EcoRII, EcoRV, HaeIII, HincIII, HindIII, HinfI, HpaI, KpnI, MspI, MciI, NcoI, NsiI, PstI, PvuII, RsaI, Sau3AI, Sau96I, ScaI, ScrFI, SphI, and others.
References

Drayna D, et al. Genetic mapping and diagnosis of haemophilia A achieved through a BclI polymorphism in the factor VIII gene. Nature 314: 738-740, 1985. PubMed: 2986011

Gitschier J, et al. Antenatal diagnosis and carrier detection of haemophilia A using factor VIII gene probe. Lancet 1: 1093-1094, 1985. PubMed: 2860301

Wood WI, et al. Expression of active human factor VIII from recombinant DNA clones. Nature 312: 330-337, 1984. PubMed: 6438526

Janco RL, et al. Improved efficacy of carrier assignment in hemophilia A using intragenic probes. Am. J. Hum. Genet. 39: A95, 1986.

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