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pH5C
pH5C
規(guī)格:
貨期:
編號:B171513
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pH5C
商品貨號 B171513
Designations pH5C
GenBank Number

M57627

Species Homo sapiens, human
Depositors DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc.
Applications
in another host, produces protein interleukin 10
Vector
Construct size (kb): 0.0
Insert
DNA: cDNA
Insert lengths(kb): 1.580000042915344
Tissue: T-lymphocyte B21 cell line, activated
Gene product: interleukin 10 [IL10]
Target Gene: interleukin 10
Insert Size (kb) 1.580
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 1.8; BglII/HindIII--3.1, 1.15, 0.91; HindIII--3.2, 1.75.
Contains the complete coding sequence. Contains 20 fewer bp in the 5' untranslated region than pH15C (ATCC 68192).
The mobility of the protein produced by COS-7 transfectants is not affected by tunicamycin.
The 3' untranslated region contains an Alu repeat. A 0.7 kb BglII/HindIII fragment from the insert is repeat-free.
The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR).
References

Brigino E, et al. Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway. Proc. Natl. Acad. Sci. USA 94: 3178-3182, 1997. PubMed: 9096366

Mosmann TR, et al. Nucleic acids encoding cytokine synthesis inhibitory factor (interleukin-10). US Patent 5,231,012 dated Jul 27 1993

Vieira P, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading fram BCRFI. Proc. Natl. Acad. Sci. USA 88: 1172-1176, 1991. PubMed: 1847510

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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