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p43-15
p43-15
規(guī)格:
貨期:
編號:B171495
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p43-15
商品貨號 B171495
Designations p43-15
Species Homo sapiens, human
Depositors LM Kunkel, SA Latt
Vector
Construct size (kb): 8.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 3.799999952316284
Tissue: lymphoblast (49,XXXXY) cell line
Gene product: DNA Segment, single copy [DXS42]
Alleles: A2, A1, C1, C2
Insert Size (kb) 3.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
A unique sequence 1.8 kb fragment is generated by PstI/HindIII double digest.
Restriction digests of the clone give the following sizes (kb): BamHI--8.2; PstI--5.1, 1.8, 0.7, 0.5; BglII--8.2; HindIII--4.4, 3.7; HindIII/BglII--4.4, 3.5; HindIII/PstI--3.6, 1.6, 1.0, 0.78, 0.5.
Linked to the Lowe oculocerebrorenal syndrome (OCRL) (theta = 0, LOD score = 6.63).
References

Aldridge J, et al. A strategy to reveal high-frequency RFLPs along the human X chromosome. Am. J. Hum. Genet. 36: 546-564, 1984. PubMed: 6328976

Wadelius C, et al. Lowe oculocerebrorenal syndrome: DNA-based linkage of the gene to Xq24-q26, using tightly linked flanking markers and the correlation to lens examination in carrier diagnosis. Am. J. Hum. Genet. 44: 241-247, 1989. PubMed: 2912070

Reilly DS, et al. Genetic and physical mapping of Xq24-q26 markers flanking the Lowe oculocerebrorenal syndrome. Genomics 8: 62-70, 1990. PubMed: 2081601

Skare JC, et al. Mapping the X-linked lymphoproliferative syndrome. Proc. Natl. Acad. Sci. USA 84: 2015-2018, 1987. PubMed: 2882515

Reilly DS, et al. Tightly linked flanking markers for the Lowe oculocerebrorenal syndrome, with application to carrier assessment. Am. J. Hum. Genet. 42: 748-755, 1988. PubMed: 2895982

Lesko JG, Nussbaum RL. RFLP locus DXS42 is proximal to the locus for hypoxanthine phosphoribosyltransferase. Am. J. Hum. Genet. 39: 669-670, 1986. PubMed: 2878611

Louis M Kunkel, personal communication

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