99精品久久99久久久久,亚洲国产成人精品无码一区二区,国产精品亚洲综合色区韩国,久久精品第一国产久精国产宅男66,亚洲国产日韩欧美一区二区三区,久久久久久精品免费无码无,国产中年熟女高潮大集合,亚洲AV无码成H在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Py230
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Py230
Py230
規(guī)格:
貨期:
編號:B171448
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Py230
商品貨號 B171448
Organism Mus musculus, mouse
Tissue mammary gland
Cell Type epithelial-like
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease adenocarcinoma
Age adult
Gender female
Applications This cell line is a model for investigating the multistep progression of malignant mammary tumorigenesis and metastasis and can also be used as a precllinical mouse model of triple negative breast cancer.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation Py230 cells were obtained from spontaneously arising tumors in MMTV-PyMY (mouse mammary tumor virus promoter driven Polyoma middle T-antigen) transgenic C57BL/6 female mice. Tumors were minced and incubated in collagenase, passed through 70 μM nylon mesh and cultured. The cells were subsequently cloned by serial trypsinization and limiting dilution.
Antigen Expression E-cadherin+ (PubMed: 24368187), cytokeratin 8+, cytokeratin 14+
Receptor Expression estrogen receptor, not expressed,
progesterone receptor, not expressed
HER2 (human epidermal growth factor receptor 2), low expression (PubMed: 22531600)
Tumorigenic yes
Effects forms luminal tumors and lung metastasis
Comments

The Py230 cell line is an epithelial-like murine mammary luminal tumor cell line that has properties similar to those of normal mouse mammary stem cells. It was derived from a mammary adenocarcinoma that spontaneously arose in a MMTV-PyMY (mouse mammary tumor virus promoter driven Polyoma middle T-antigen) transgenic C57BL/6 female mouse. This cell line carries the Polyoma virus middle T oncogene. (L Ellies, personal communication)

Py230 cells are cuboidal-shaped and grow in well-differentiated colonies at confluence in culture. (PubMed:  24368187)

This cell line forms luminal tumors and lung metastasis in vivo. (Deposit Form)

Py230 cells differentiate into single positive luminal, myoepithelial, and alveolar cells upon stimulation with lactogenic hormones. (L Ellies, personal communication)

Py230 cells express luminal-epithelial markers E-cadherin, cytokeratin 8 and cytokeratin14. Py230 cells are negative for expression of estrogen receptor, progesterone receptor and express low levels of HER2. (PubMed:  24368187) 

The Py230 cell line has a more differentiated phenotype and grows less aggressively in cell culture in comparison to the undifferenitiated and mesenchymal CRL-3280, Py8119 cells, which were also derived from a MMTV-PyMY transgenic C57BL/6 female mouse. (PubMed:  22531600)

Py230 cells, along with Py8119 cells, are a pair of murine mammary cell lines with distinct epithelial-like (Py230) or mesenchymal (Py8119) features derived from MMTV-PyMT transgene-induced mammary tumors in C57BL/6 mice that can be useful to study mammary tumorigenesis. (PubMed:  24368187) 

Complete Growth Medium The base medium for this cell line is F-12K Medium (ATCC 30-2004). To make the complete growth medium, add the following component to the 500 mL of the base medium:
  • Fetal bovine serum (FBS; ATCC 30-2020) for a final concentration of 5%
  • MITO+ Serum Extender (Corning? #355006) for a final concentration of 0.1%
Subculturing

Note: these cells will not maintain their differentiation properties without MITO+ Serum Extender.

Note: these cells grow slowly

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.25% Trypsin – 2.21mM EDTA in HBSS (Corning cat no. 25-053-Cl) solution to remove all traces of serum which contains trypsin inhibitor.

2. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note:  These cells will form small clumps upon trypsinization.They do not form a single cell suspension. To avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. An inoculum of 3 X 104 to 5 X 104 viable cells/cm2 is recommended.

4. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subculture when cell density reaches between 2 X 105 and 3 X 105 cells/cm2.

 

 

Subcultivation Ratio: 1:3 to 1:6 is recommended.

Medium renewal: every other day

 

Note: These cells will differentiate if maintained at low density.

Note: It is recommended to make clones from early passages. Subclone by plating into ultra-low adhesion plates at low density for 2 days. Lightly trypsinize cells and pick single cells, place into wells of 96-well plate with 150µL complete medium. Leave in incubator for 2 weeks. Check for colony growth. Keep only colonies that grow and form domes.
Cryopreservation Freeze Medium: Complete culture medium + 40% additional FBS (ATCC 30-2020) + 10% DMSO (ATCC 4-X)
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Volume 1.0 mL
Name of Depositor L Ellies, University of California, San Diego
Year of Origin June 2004
References

Guy CT, et al. Induction of mammary tumors by expression of polyomavirus middle T oncogene: a transgenic mouse model for metastatic disease. Mol. Cell Biol. 12: 954-961, 1992. PubMed: 1312220

Maglione JE, et al. Transgenic Polyoma middle-T mice model premalignant mammary disease. Cancer Res. 61: 8298-8305, 2001. PubMed: 11719463

Gibby K, et al. Early vascular deficits are correlated with delayed mammary tumorigenesis in the MMTV-PyMT transgenic mouse following genetic ablation of the NG2 proteoglycan. Breast Cancer Res. 14: R67, 2012. PubMed: 22531600

Biswas T, et al. Attenuation of TGF-β signaling supports tumor progression of a mesenchymal-like mammary tumor cell line in a syngeneic murine model. Cancer Lett 346: 129-138, 2014. PubMed: 24368187

Bao L, et al. Multipotent luminal mammary cancer stem cells model tumor heterogeneity. Breast Cancer Res. 17(1): 137, 2015. PubMed: 26467658

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
三门县| 九寨沟县| 资源县| 慈溪市| 岳阳县| 青神县| 广西| 永昌县| 黔南| 肇州县| 朝阳县| 措勤县| 珲春市| 莆田市| 汉阴县| 神池县| 柯坪县| 衡南县| 四川省| 从化市| 仁化县| 鄂伦春自治旗| 尼勒克县| 旺苍县| 四会市| 通化市| 固原市| 靖西县| 舟山市| 即墨市| 始兴县| 延长县| 贵州省| 宜章县| 锦州市| 林州市| 永泰县| 明星| 延边| 新巴尔虎右旗| 蚌埠市|