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Stephanopogon apogon Borror
Stephanopogon apogon Borror
規(guī)格:
貨期:
編號:B171416
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Stephanopogon apogon Borror
商品貨號 B171416
Strain Designations BCP
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
seawater, Cliff Pool, Bermuda, 1984
Product Format frozen
Storage Conditions Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic Xenic
Type Strain no
Comments
Food source, Rhynchomonas nasuta ATCC 50105, not supplied. Mixed bacteria present.
Ultrastructural identity
Medium ATCC® Medium 1525: Seawater Sonneborn's Paramecium Medium
ATCC® Medium 1405: HESNW medium
ATCC® Medium 1361: Marine flagellate medium
Growth Conditions
Temperature: 25°C
Cryopreservation

Cryoprotective Solution
DMSO, 2.0 ml
Fresh growth medium w/o bacteria, 8.0 ml

  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest Stephanopogon cells or cysts from a culture that has recently passed peak density by filtration and centrifugation at 800 x g for 5 min.
  3. Adjust the concentration of cysts at least 2 x 105/ml in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml of an equal-parts mixture of ATCC medium 1525 and either ATCC medium 1405 or ATCC medium 1361, bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC® 700831?) or Enterobacter aerogenes (ATCC® 13048™).
  9. Aseptically transfer 1-2 ml from a thriving culture of Rhynchomonas to the T-25 flask. Incubate the culture at 25°C with the cap screwed on tightly.

Once the culture is established, follow the protocol for maintenance of culture.

Name of Depositor EB Small
Year of Origin 1984
References

Patterson DJ, Brugerolle G. The ultrastructural identity of Stephanopogon apogon and the relatedness of the genus to other kinds of protists. Eur. J. Protistol. 23: 279-290, 1988.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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