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<em>Penicillium rubens</em> Biourge
<em>Penicillium rubens</em> Biourge
規(guī)格:
貨期:
編號(hào):B170649
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Penicillium rubens Biourge
商品貨號(hào) B170649
Deposited As Penicillium chrysogenum Thom
Strain Designations WIS 54-1255 [Wisconsin 54-1255; Wis. 51-20; Wis. 48-70; NRRL 1951; ATCC 9480]
Application
Produces acyl-CoA-6-aminopenicillanic acid acyltransferase
Produces isopenicillin N synthetase
Produces penicillin
Produces penicillin V
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol&reg; no
Genome Sequenced Strain

Yes

Comments
High penicillin producer
Phenylacetic acid transport system
Genome sequencing strain (DSM Anti-Infectives, The Netherlands).
The strain was reclassified as Penicillium rubens based on the multigene sequence analysis.
Morphology After 5-6 days at 25°C on potato dextrose medium colonies growing rapidly, velutinous to floccose. Conidiophore stipes smooth-walled, 200-300 μm long; penicilli usually terverticillate. Metulae 8-12 μm long. Phialides flask-shaped, 7-10 μm long. Conidia smooth-walled, ellipsoidal, 2.5-4.0 μm long, blue or bluish-green.
Medium ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 325: Malt extract agar (Blakeslee's formula)
ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCCAACCTCCCACCCGTGTTTATTTTACCTTGTTGCTTCGGCGGGCCCGCCTTAACTGGCCGCCGGGGGGCTTACGCCCCCGGGCCCGCGCCCGCCGAAGACACCCTCGAACTCTGTCTGAAGATTGTAGTCTGAGTGAAAATATAAATTATTTAAAACTTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTTCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCGTCCTCCGATCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTTGTCACCCGCTCTGTAGGCCCGGCCGGCGCTTGCCGATCAACCCAAATTTTTATCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGTAGAGGATGCTTCGGGAGCGGTCCCCATCTAAGTGCCCTGGAACGGGACGTCATAGAGGGTGAGAATCCCGTATGGGATGGGGTGTCCGCGCCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTCGCTCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGCGGGCGGGCCAGCGTCGGTTTGGGCGGTCGGTCAAAGGCCCTCGGAAGGTAACGCCCCTAGGGGCGTCTTATAGCCGAGGGTGCAATGCGACCTGCCTAGACCGAGGAACGCGCTTCGGCTCGGACGCTGGCATAATGGTCGTAAACGAC


beta-tubulin gene

GATCCCCTCCAGACGCGTCTTTTGTTTTCATATGGCCCCTGAGCCACGACCCCACCCCCAACAGATCTTTTGCTAACTTGCTCTAGGTTCACCTCCAAACCGGCCAGTGTGTAAGTTCAACATGGAACATTCTTGGAAACATTCTTCGATTCGTGGGACTAAACTGGAATTGGGTTATAGGGTAACCAAATTGGTGCCGCTTTCTGGTAAGTCTCGGAGCTTTTTTTTTCGCGTTGGGTATCAATTGACAAGTTGCTAACTGGATTACAGGCAAACCATCTCTGGCGAGCACGGTCTCGATGGCGATGGACAGTAAGTTTAACAGTGATAGGGATTCTGGTGGATCACACGTCTGATATCTTGCTAGGTACAATGGTACCTCCGACCTCCAGCTCGAGCGTATGAACGTCTACTTCAACCATGTGAGTACAATGACTGGGAATCGATTGATTGTGCATCATCTGATCGGACGTTTTTCTTTGACAATCTAGGCCAGCGGTGACAAGTACGTTCCCCGTGCCGTTCTGGTCGATTTGGAGCCCGGTACCATGGATGCTGTCCGCTCCGGTCCCTTCGGCAAGCTTTTCCGCCCCGACAACTTCGTCTTCGGTCAGTCCGGTGCTGGTAACAACTGG
Name of Depositor CY O'Sullivan
Chain of Custody
ATCC <-- CY O'Sullivan <-- Wis. 51-20 <-- Wis. 48-70 <-- NRRL 1951
References

O'Sullivan CY, Pirt SJ. Penicillin production by lysine auxotrophs of Penicillium chrysogenum. J. Gen. Microbiol. 76: 65-75, 1973. PubMed: 4198715

Luengo JM, et al. Direct enzymatic synthesis of penicillin G using cyclases of Penicillium chrysogenum and Acremonium chrysogenum. Bio-Technology 4: 44-47, 1986.

Ramsay BA, et al. Penicillin production in an inverse fluidized bed bioreactor. J. Ferment. Bioeng. 72: 495-497, 1991.

Ferrero MA, et al. Biosynthesis of benzylpenicillin (G), phenoxymethylpenicillin (V) and octanoylpenicillin (K) from glutathione S-derivatives. J. Antibiot. 43: 684-691, 1990. PubMed: 2166024

Martin-Villacorta J, et al. Acyl-CoA: 6-APA acyltransferase from Penicillium chrysogenum studies on its hydrolytic activity. J. Antibiot. 44: 108-110, 1991. PubMed: 1848214

Ferrero MA, et al. In vitro enzymatic synthesis of new penicillins containing keto acids as side chains. Antimicrob. Agents Chemother. 35: 1931-1932, 1991. PubMed: 1952871

Alonso MJ, et al. Enzymatic synthesis of penicillins. J. Antibiot. 41: 1074-1084, 1988. PubMed: 3170343

Fernandez-Canon JM, et al. Uptake of phenylacetic acid by Penicillium chrysogenum Wis 54-1255: a critical regulatory point in benzylpenicillin biosynthesis. J. Antibiot. 42: 1398-1409, 1989. PubMed: 2507493

Fernandez-Canon JM, et al. Phenylacetic acid transport system in Penicillium chrysogenum Wis 54- 1255: molecular specificity of its induction. J. Antibiot. 42: 1410-1415, 1989. PubMed: 2507494

Martinez-Blanco H, et al. Repression of phenylacetic acid transport system in Penicillium chrysogenum Wis 54-1255 by free amino acids and ammonium salts. J. Antibiot. 42: 1416-1423, 1989. PubMed: 2507495

MacDonald KD, et al. Properties of heterozygous diploids between strains of Penicillium chrysogenum selected for high penicillin yield. Antonie van Leeuwenhoek 30: 209-224, 1964.

Xu Z, et al. Genome physical mapping from large-insert clones by fingerprint analysis with capillary electrophoresis: a robust physical map of Penicillium chrysogenum. Nucleic Acids Res. 33: e50, 2005. PubMed: 15767275

van den Berg MA, et al. Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum. Nat. Biotechnol. 26:1161-1168, 2008. PubMed: 18820685

Houbraken J, et al. New penicillin-producing Penicillium species and an overview of section Chrysogena. Persoonia 29: 78-100, 2012. PubMed: 23606767

Hoff B, Poggeler S, Kuck U. Eighty years after its discovery, Fleming's Penicillium strain discloses the secret of its sex. Eukaryot Cell 7: 465-470, 2008. PubMed: 18223118

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