產(chǎn)品名稱 |
Listeria monocytogenes |
商品貨號(hào) |
B96997 |
中文名稱 |
單核增生李斯特菌 |
其它保藏中心編號(hào) |
BCRC14848,ATCC 19114 ;CCM 5579 ;CIP 78.37 ;NCTC 5214 |
特征特性 |
Nucleotide (GenBank) : X85881 L. monocytogenes type 3 partial iap gene (strain ATCC 19114).Nucleotide (GenBank) : U84148 Listeria monocytogenes 16S ribosomal RNA gene, partial sequence. Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)Jones FS, Little RB. . Arch. Pathol. 18: 580, 1934.Paterson S. . J. Pathol. Bacteriol. 51: 427-436, 1940.Seeliger HPR. Listeriosis, 2nd ed.. New York: Karger; 1961. |
具體用途 |
藥敏質(zhì)控、培養(yǎng)基質(zhì)控科研,生產(chǎn),主要用于腸內(nèi)研究 |
培養(yǎng)基 |
ATCC? Medium 44: Brain Heart Infusion Agar/Broth ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
分離基物 |
組織,動(dòng)物 |
凍干物說明 |
·凍干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培養(yǎng)液或者無菌水溶解,接種在1-2個(gè)平板上,因凍干菌種處于休眠狀態(tài),請(qǐng)勿接種多個(gè)平板,以免因接種量不足而導(dǎo)致復(fù)蘇不成功; |
保存說明 |
1、長(zhǎng)期保存的儲(chǔ)存菌株以冷凍干燥保藏為主,特殊要求的菌種可用其它方法進(jìn)行保存,保存期可長(zhǎng)達(dá)幾年至幾十年。2、半儲(chǔ)存菌株以含20%甘油的肉湯或液體石臘封存的半固體瓊脂為主,在0℃以下保存,保存期為3-6個(gè)月。3、應(yīng)用菌株以瓊脂斜面為主,在4℃保存,保存期為1-3個(gè)月。4、菌種的傳代不可超過6次。視菌種的特性及使用情況,一般儲(chǔ)存菌株每5年傳記代一次,每1-2年從儲(chǔ)存菌株轉(zhuǎn)接至半儲(chǔ)存菌株,半儲(chǔ)存菌株每3-6個(gè)月傳代一次,每1-3個(gè)月從半儲(chǔ)存菌株轉(zhuǎn)接接至應(yīng)用菌株。 |