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pDP252
pDP252
規(guī)格:
貨期:
編號(hào):B241817
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pDP252
商品貨號(hào) B241817
Designations pDP252
Species Hepatitis B virus, Hepatitis B virus
Depositors Health Research, Inc., D Panicali, Health Research, Inc.
Applications
This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims.
Vector
Construct size (kb): 5.440000057220459
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: hepatitis B virus
Gene symbol: sAg
Gene name: surface antigen
Contains complete coding sequence?: U
Type of DNA: unknown
Insert end: HhaI
Insert end: HhaI
Insert size (kb): 1.08
Cross references:
Insert lengths(kb): 1.080000042915344
Gene product: surface antigen [sAg]
Insert Size (kb) 1.080
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
The insert contains a BamHI site.
Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 1.15; EcoRI--5.7.
The insert contains a BamHI site. This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims.
Constructed by cloning a 1.084 kb HhaI fragment of HBV into pBR322
This clone was constructed by isolating a 1.084 kb HhaI fragment from pTHBV containing the coding sequence for the surface antigen without pre-surface antigen. This was blunt-ended with T4 DNA polymerase, linkered with HindIII, and inserted into pBR322.
Classification Hepadnaviridae, Orthohepadnavirus
References

Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986

Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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