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The recommended probe is the 0.76 kb EcoRI/HindIII exon 2 fragment.

Construct size (kb): 9.0

DNA: genomic

Insert lengths(kb): 7.0

Tissue: HL-60 cell line (promyelocytic leukemia)

Gene product: neuroblastoma RAS viral (v-ras) oncogene homolog( v-ras neuroblastoma RAS viral oncogene homolog, proto-oncogene N-ras) [NRAS]

Alleles: A1, A2

Restriction digests of the clone give the following sizes (kb): EcoRI--5.5, 3.5; PvuII--4.2, 3.4, 1.0, 0.6.

The insert can transform NIH 3T3 cells. The recommended probe is the 0.76 kb EcoRI/HindIII exon 2 fragment.

Contains pBR322 (EcoRI/PvuII, 2.05 kb); SV40 ori (PvuII/HindIII, 344 bp); exon 1 (HindIII/HindIII); exon 2 (HindIII/EcoRI, 760 bp); exon 3/exon 4 (EcoRI/SacI, 5 kb), and SV40 polyadenylation signal (SacI/EcoRI).