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Trypanosoma cruzi Chagas
Trypanosoma cruzi Chagas
規(guī)格:
貨期:
編號:B234520
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 Trypanosoma cruzi Chagas
商品貨號 B234520
Strain Designations WA250 CL2
Application
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
clone 2 derived from strain WA-250 originally isolated from an opposum, Didelphis abiventris, 1977
Product Format frozen
Type Strain no
Medium ATCC® Medium 1029: LIT medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Cryopreservation
1.?? Harvest cells from several cultures in very late logarithmic to early stationary phase of growth.? Vigorously agitate to suspend the cells.

2.?? Aseptically transfer the cell suspension to 15 ml plastic centrifuge tubes.

3.?? Centrifuge at ~800 x g for 5 min.

4.?? While cells are centrifuging, prepare a 10% solution of DMSO in complete ATCC Medium 1029.? Cool on ice.

5.?? Remove the supernatant and pool the cell pellets to the final volume desired with fresh growth medium.

6.?? Combine the cell suspension with an equal volume of 10% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 5% DMSO.

7.?? Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).

8.?? Place the vials in a controlled rate freezing unit.? From room temperature cool at -1°C/min to -40°C.? At -40°C, plunge ampules into liquid nitrogen.? Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.). ?

9.?? Store ampules in a liquid nitrogen refrigerator until needed.

10.????????? To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.? Allow the ampule to thaw completely (2-3 min).

11.????????? Immediately after thawing, aseptically remove the contents and transfer to a T-25 tissue culture flask containing 10 ml of fresh complete ATCC medium 1029.

12.Screw the cap on tightly and incubate at 20-25°C.??? Observe the culture daily and transfer when numerous trophozoites are observed.?????????

Name of Depositor JA Dvorak
Special Collection NCRR Contract
References

Dvorak JA, et al. Trypanosoma cruzi: flow cytometric analysis. I. Analysis of total DNA/organism by means of mithramycin-induced fluorescence. J. Protozool. 29: 430-437, 1982. PubMed: 6182288

Bongertz V, Dvorak JA. Trypanosoma cruzi: antigenic analysis of cloned stocks. Am. J. Trop. Med. Hyg. 32: 716-722, 1983. PubMed: 6349399

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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