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pBSV2
pBSV2
規(guī)格:
貨期:
編號(hào):B214536
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pBSV2
商品貨號(hào) B214536
Designations pBSV2
Depositors PE Stewart
Biosafety Level 1
Host
Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 6.4000000953674320
DESCRIPTION OF VECTOR:
Intact vector size: 6.400
Type of vector: plasmid
Cloning sites: NotI SacI EcoRI KpnI BamHI XbaI SalI PstI SphI HindIII NotI
Polylinker sites: NotI SacI EcoRI KpnI BamHI XbaI SalI PstI SphI HindIII NotI
Other unique sites: NcoI SfiI SpeI
Construction: pCR-XL-TOPO
Features (with orientation and position when available):
primer site: M13 (forward, reverse)
promoter: lacZalpha
replicon: ColE1, ORFs
marker(s): kanamycin, zeocin
Vector: pBSV2 (plasmid)
Promoters: Promoter lacZalpha
Construction: pCR-XL-TOPO
Marker(s):kanR
Construct size (kb): 6.400000095367432
Features: marker(s): kanamycin, zeocin
primer site: M13 (forward, reverse)
promoter: lacZalpha
replicon: ColE1, ORFs
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.4; EcoRI--4.2, 2.2; HindIII--6.4.
The plasmid pBSV2 is a shuttle vector capable of moving between E. coli and the Lyme disease spirochete, Borrelia burgdorferi.
Growth Conditions
Temperature: 37.0°C
References

Stewart P, et al. Isolation of a circular plasmid region sufficient for autonomous replication and transformation of infectious Borrelia burgdorferi. Mol. Microbiol. 39: 714-721, 2001. PubMed: 11169111

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