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<i>Clostridioides difficile</i> (Prevot) Lawson et al.
<i>Clostridioides difficile</i> (Prevot) Lawson et al.
規(guī)格:
貨期:
編號(hào):B166176
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

規(guī)格:
凍干粉
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甘油
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產(chǎn)品名稱 Clostridioides difficile (Prevot) Lawson et al.
商品貨號(hào) B166176
Deposited As Clostridium difficile (Hall and O'Toole) Prevot
Strain Designations 630
Application
Enteric Research
Isolation
Clincal isolate, Switzerland
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol&reg; no
Genotype Toxinotype: 0 RefRupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Genome Sequenced Strain

Clostridium difficile 630, complete genome

Type Strain no
Comments
Genome sequenced strain
Presence of tcdA and tcdB genes confirmed by PCR. RefLemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Binary toxin gene cdtB not amplified by PCR
Ribotype 012
Toxinotype 0
Medium ATCC® Medium 2107: Modified Reinforced Clostridial
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor B Wren, H Maschler
References

Wust Jurg, et al. Investigation of an Outbreak of Antibiotic-Associated Colitis by Various Typing Methods. J. Clin. Microbiol. 16: 1096-1101, 1982.

Sebaihia Mohammed. The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat. Genet. 38: 779-786, 2006.

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Cross References

Nucleotide (Genbank) : NC_009089 Clostridium difficile 630, complete genome

Nucleotide (GenBank) : NC_008226 Clostridium difficile 630 plasmid pCD630, complete sequence.

Nucleotide (GenBank) : AM180355 Clostridium difficile 630 complete genome.

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