| 產品名稱 | ZF4 |
|---|---|
| 商品貨號 | B165992 |
| Organism | Danio rerio, zebrafish |
| Tissue | embryo |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 1 day old embryo |
| Applications | The cells can be used as a transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | hyperploid; range = 110 to 120 |
| Images |  |
| Derivation | The ZF4 cell line was established from 1-day-old zebrafish embryos. |
| Comments | The ZF4 cell line was established from 1-day-old zebrafish embryos. The cells are cultivated at 28°C.
ZF4 cells are killed within 10 days by culturing in medium containing 0.8 to 1.0 mg/mL G418. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
NOTE: DO NOT USE EDTA TO DISSOCIATE THE CELLS. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedMedium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 28°C |
| Name of Depositor | W Driever |
| Deposited As | Brachydanio rerio |
| References | Driever W, Rangini Z. Characterization of a cell line derived from zebrafish (Brachydanio rerio) embryos. In Vitro Cell. Dev. Biol. Anim. 29A: 749-754, 1993. PubMed: 8407719 Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
