| 產(chǎn)品名稱 |
SV63 |
| 商品貨號 |
B165766 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Choriocarcinoma |
| Strain |
(BALB/c X C57BL/6)F1 (B cell); BALB/c (myeloma) |
| Applications |
The SV63 antibody reacts with an epitope present on the placental, intestinal, and germ cell forms of human alkaline phosphatase, but not on the liver/kidney/bone form of the enzyme. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human choriocarcinoma tumor cell antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human choriocarcinoma tumor cell antigen |
| Comments |
Animals were immunized with the GCC-SV(c) human choriocarcinoma cell line. Spleen cells were fused with NS-1 myeloma cells. The SV63 antibody reacts with an epitope present on the placental, intestinal, and germ cell forms of human alkaline phosphatase, but not on the liver/kidney/bone form of the enzyme. The antibody precipitates a glycoprotein about 68000 daltons from extracts of choriocarcinoma cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- HT
- 1 mM non-essential amino acids
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.
Maintain cell density between 1 X 105 and 1 X 106 viable cells/ml.
Growth is enhanced by using a feeder layer of irradiated STO cells. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
Sloan-Kettering Institute for Cancer Research |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Rettig WJ, et al. Choriocarcinoma monoclonal antibodies and antibody panels. US Patent 4,851,332 dated Jul 25 1989
|
