| 產(chǎn)品名稱 | SK-N-FI |
|---|---|
| 商品貨號 | B165717 |
| Organism | Homo sapiens, human |
| Tissue | brain; derived from metastatic site: bone marrow |
| Cell Type | neuroblast |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | neuroblastoma |
| Age | 11 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | SK-N-FI is a neuroblastoma cell line derived in 1979 from a bone marrow metastasis from a 11 year old Caucasian male with poorly differentiated embryonal neuroblastoma. |
| Clinical Data | 11 years
Caucasian
male
|
| Receptor Expression | tumor necrosis factor (TNF) type B, expressed tumor necrosis factor (TNF) type A, expressed |
| Genes Expressed | tumor necrosis factor (TNF) |
| Cellular Products | tumor necrosis factor (TNF) |
| Tumorigenic | Yes |
| Effects | Yes, forms tumors in nude mice with a latent period of 4 to 6 weeks |
| Comments | The cells exhibit high MDR1 expression.
Recombinant tumor necrosis factor (TNF) stimulates the proliferation of SK-N-FI cells in both serum free medium and fetal bovine serum supplemented medium, but has no effect in medium without insulin. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,14 D16S539: 11,12 D5S818: 12,13 D7S820: 8,9 THO1: 6,9.3 TPOX: 8,9 vWA: 16,17 |
| Name of Depositor | C Helson |
| Deposited As | Homo sapiens |
| Year of Origin | 1979 |
| References | Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792 Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824 Goillot E, et al. Tumor necrosis factor as an autocrine growth factor for neuroblastoma. Cancer Res. 52: 3194-3200, 1992. PubMed: 1317260 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
