| 產(chǎn)品名稱 |
SK-MEL-28 |
| 商品貨號 |
B165708 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Cell Type |
Melanoma |
| Product Format |
frozen |
| Morphology |
polygonal |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
malignant melanoma |
| Age |
51 years adult |
| Gender |
male |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell. |
| Derivation |
|
| Clinical Data |
male |
| Antigen Expression |
Antigen expression: Blood Type A; Rh+; HLA A11, A26, B40, DRw4 |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice; forms malignant melanoma (large round cell type) |
| Comments |
This is one of a very extensive series of melanoma lines (see also ATCC HTB-70, ATCC HTB-71 and ATCC HTB-73) isolated by T. Takahashi and associates. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,12 D16S539: 9,12 D5S818: 11,13 D7S820: 10,9.3 THO1: 7 TPOX: 8,12 vWA: 16,19 |
| Isoenzymes |
AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1 |
| Name of Depositor |
T Takahashi |
| Deposited As |
Homo sapiens |
| References |
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619
Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988
Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.
|
| Cross References |
Nucleotide (GenBank) :
D55649
Human mRNA for alpha mannosidase II isozyme, complete cds.
Nucleotide (GenBank) :
L28821
Homo sapiens alpha mannosidase II isozyme mRNA, complete cds.
Nucleotide (GenBank) :
NM_006122
Homo sapiens mannosidase, alpha, class 2A, member 2 (MAN2A2), mRNA.
Nucleotide (GenBank) :
AW067366
EST00008 RT-PCR products human melanoma Homo sapiens cDNA clone Mel8, mRNA sequence.
Nucleotide (GenBank) :
AW067367
EST00009 RT-PCR products human melanoma Homo sapiens cDNA clone Mel10, mRNA sequence.
Nucleotide (GenBank) :
AW067368
EST00010 RT-PCR products human melanoma Homo sapiens cDNA clone Mel12, mRNA sequence.
Nucleotide (GenBank) :
AW067369
EST00011 RT-PCR products human melanoma Homo sapiens cDNA clone Mel15, mRNA sequence.
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