| 產(chǎn)品名稱 |
Ramos.2G6.4C10 |
| 商品貨號 |
B165573 |
| Organism |
Homo sapiens, human |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Burkitt's lymphoma (American) |
| Age |
3 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
Ramos.2G6.4C10 cells provide a sensitive and specific assay system for IL-4 that is insensitive to the presence of other lymphokines and cytokines such as interleukins (IL-2, IL-2 and IL-6), colony stimulating factors (G-CSF, M-CSF and GM-CSF) and interferons alpha and gamma (IFN alpha, IFN gamma). Tumor necrosis factors alpha and beta (TNF alpha, TNF beta) cause only minor interference.
The assay is sensitive to IL-4 concentrations in the range 50 to 800 pg/mL. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line was derived from the Ramos (RA-1) cell line (see ATCC CRL-1596) by repetitive cloning with selection for low constitutive expression of CD23 and maximum stimulation of CD23 expression by IL-4. |
| Clinical Data |
3 years Caucasian male |
| Antigen Expression |
CD23 + |
| Receptor Expression |
interleukin 4 (IL-4); low affinity IgE (CD23) |
| Genes Expressed |
immunoglobulin (surface and secreted) |
| Cellular Products |
immunoglobulin (surface and secreted) |
| Comments |
Both the parental line (ATCC CRL-1596) and the derivative cell line Ramos.2G6.4C10 (ATCC CRL-1923) have about 1500 IL-4 binding sites per cell.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 x 10 5 viable cells/mL and maintain between 1 and 2 x 10 6 cells/mL.
Medium Renewal: Add fresh medium every 4 to 6 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 10,13 D5S818: 7,12 D7S820: 11 THO1: 7,9.3 TPOX: 8,9 vWA: 15,16 |
| Isotype |
IgM |
| Name of Depositor |
JP Siegel |
| Deposited As |
Homo sapiens |
| References |
Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343
Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879
Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726
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