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Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 3 to 4 x 10⁴ viable cells/mL. Do not allow the cell concentration to exceed 5 x 10⁵ cells/mL.

Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Temperature: 37°C 
Atmosphere: 10% CO₂

Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164

Coffman RL, Weissman IL. B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681-683, 1981. PubMed: 6970340

Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.