99精品久久99久久久久,亚洲国产成人精品无码一区二区,国产精品亚洲综合色区韩国,久久精品第一国产久精国产宅男66,亚洲国产日韩欧美一区二区三区,久久久久久精品免费无码无,国产中年熟女高潮大集合,亚洲AV无码成H在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > R4-6A2
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
R4-6A2
R4-6A2
規(guī)格:
貨期:
編號:B165562
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 R4-6A2
商品貨號 B165562
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast
Culture Properties Suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody neutralizes the ability of lymphokine preparations to induce tumoricidal activity in murine macrophages. 

The antibody neutralizes the antiviral effect of gamma interferon but does not affect the activities of murine alpha and beta interferon or rat or human gamma interferon.

30 ng of R4-6A2 antibody neutralizes the antiviral activity of 10 units of murine gamma interferon.

Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with partially purified murine gamma interferon. 

Spleen cells were fused with P3U-1 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against mouse gamma interferon
Cellular Products
immunoglobulin; monoclonal antibody; against mouse gamma interferon
Tumorigenic Yes
Effects

Forms ascites in nude mice.

Comments

The antibody neutralizes the ability of lymphokine preparations to induce tumoricidal activity in murine macrophages. 

The antibody neutralizes the antiviral effect of gamma interferon but does not affect the activities of murine alpha and beta interferon or rat or human gamma interferon.

30 ng of R4-6A2 antibody neutralizes the antiviral activity of 10 units of murine gamma interferon.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 viable cells/mL. Maintain cultures at a cell concentration between 6 x 104 and 6 x 105 cells/mL. Do not allow the cell concentration to reach 1 x 106 cells/mL.

Medium Renewal: Every 2 to 3 days
Note: Do not allow the cell concentration to reach 1 x 106 cells/mL.
Cryopreservation
Complete culture medium, 92.5%; DMSO, 7.5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor GL Spitalny
Deposited As rat (B cell); mouse (myeloma)
References

Spitalny GL, Havell EA. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. J. Exp. Med. 159: 1560-1565, 1984. PubMed: 6425450

Tay CH, Welsh RM. Distinct organ-dependent mechanisms for the control of murine cytomegalovirus infection by natural killer cells. J. Virol. 71: 267-275, 1997. PubMed: 8985346

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Skeen MJ, et al. Regulation of murine macrophage IL-12 production. J. Immunol. 156: 1196-1206, 1996. PubMed: 8557998

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
乐业县| 勐海县| 长沙县| 新巴尔虎右旗| 大丰市| 绩溪县| 蓬莱市| 固镇县| 满城县| 蚌埠市| 红安县| 丹阳市| 新泰市| 罗甸县| 宁乡县| 清水县| 成安县| 巴彦县| 顺平县| 闽侯县| 来宾市| 河北省| 南丹县| 丰县| 宾阳县| 赤壁市| 陆川县| 兴和县| 加查县| 静宁县| 博罗县| 藁城市| 图木舒克市| 乐陵市| 吉林省| 沙坪坝区| 武功县| 上饶市| 南华县| 岢岚县| 沛县|