99精品久久99久久久久,亚洲国产成人精品无码一区二区,国产精品亚洲综合色区韩国,久久精品第一国产久精国产宅男66,亚洲国产日韩欧美一区二区三区,久久久久久精品免费无码无,国产中年熟女高潮大集合,亚洲AV无码成H在线观看

Subcultures are prepared by scraping. Remove one-half of the old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Mizel SB, et al. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line P388D1. I. Enhancement of LAF production by activated T lymphocytes. J. Immunol. 120: 1497-1503, 1978. PubMed: 149171

Evans VJ, et al. A new tissue culture isolation and explanation of the P388 lymphocytic neoplasm in a chemically characterized medium. Exp. Cell Res. 32: 212-217, 1963. PubMed: 14094040

Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231

Dawe CJ, Potter M. Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33: 603, 1957

Rabson AS, Leggallais FY. Cytopathogenic effect produced by polyoma virus in cultures of milk-adapted murine lymphoma cells (strain P388 D1). Proc. Soc. Exp. Biol. Med. 100: 229-233, 1959. PubMed: 13634094

. . Nature 181: 1343, 1958.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.