| 產(chǎn)品名稱 |
NCI-H522 [H522] |
| 商品貨號(hào) |
B165350 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent, single cells and loosely attached clusters |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage 2,adenocarcinoma; non-small cell lung cancer |
| Age |
58 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 53. This is a hypotriploid human cell line with the modal chromosome number occurring in 68% of cells counted. The polyploid cells occurred at 3.0%; eleven marker chromosomes were common to all cells, of which two were paired. The markers included: der(6)(q13), t(7p17q), del(10)(p11.23), paired der(7)t(7;?17)(p22;?q21.1) and der(16)t(16;?)(q24;?), and six others; normal Y, N13, N15, and N16 were absent; the X chromosomes were mostly paired. |
| Derivation |
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lung cancer obtained from a patient prior to therapy.- |
| Clinical Data |
58 years
Caucasian
male
The patient was a smoker (60-pack-year).
|
| Comments |
The cells carry a mutation in codon 191 of the TP53 gene.
The cells are not able to synthesize the peptide neuromedin B (NMB) or the gastrin releasing peptide (GRP).
The cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 10 D16S539: 12 D5S818: 11 D7S820: 9,10 THO1: 7,9.3 TPOX: 9,10 vWA: 17 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| References |
Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
Mitsudomi T, et al. p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features. Oncogene 7: 171-180, 1992. PubMed: 1311061
Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
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