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NCI-H1930 [H1930]
NCI-H1930 [H1930]
規(guī)格:
貨期:
編號(hào):B165296
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 NCI-H1930 [H1930]
商品貨號(hào) B165296
Organism Homo sapiens, human
Tissue lung
Product Format frozen
Culture Properties suspension, multicell aggregates
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease stage L, carcinoma; classic small cell lung cancer
Age 41 years
Gender male
Ethnicity White
Storage Conditions liquid nitrogen vapor phase
Karyotype del(p13-p21)
Derivation
The line was established in May 1988 from lymph node, a metastatic site.
Clinical Data
The tissue donor was a non-smoker.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cells grow as floating aggregates of round cell clusters. Clusters are alive while single cells have very low viability. Do not try to break up the clusters as this would decrease the viability. Centrifuge cell suspension every 2 to 3 days. After centrifugation, aspirate supernatant down to 1 mL above cell pellet. Resuspend cells by gentle swirling in 3 to 4 mL of fresh medium. Seed at a concentration of 2 to 2.5 x 104 viable cells/mL. Transfer to new culture flasks when necessary.
Cryopreservation
50% FBS + 40% Culture Media + 10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
Year of Origin 1988
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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