| 產(chǎn)品名稱 |
NCI-H1404 [H1404] |
| 商品貨號(hào) |
B165262 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
suspension, multicell aggregates |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
papillary adenocarcinoma |
| Age |
48 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy. |
| Clinical Data |
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy. male Caucasian 48 years |
| Genes Expressed |
surfactant associated protein A (SP-A); surfactant associated protein B (SP-B) |
| Cellular Products |
surfactant associated protein A (SP-A); surfactant associated protein B (SP-B) |
| Tumorigenic |
Yes |
| Effects |
Yes, the cells produce tumors in athymic nude mice |
| Comments |
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. The cells express protein and RNA of SP-A, the major surfactant associated protein, and SP-B. SP-C RNA was not expressed. |
| Complete Growth Medium |
The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%. |
| Subculturing |
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 10% fetal bovine serum and 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 9,14 D16S539: 12 D5S818: 12 D7S820: 11,14 THO1: 7,9.3 TPOX: 9,11 vWA: 19 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| References |
Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
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