99精品久久99久久久久,亚洲国产成人精品无码一区二区,国产精品亚洲综合色区韩国,久久精品第一国产久精国产宅男66,亚洲国产日韩欧美一区二区三区,久久久久久精品免费无码无,国产中年熟女高潮大集合,亚洲AV无码成H在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > M/K-2.7
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
M/K-2.7
M/K-2.7
規(guī)格:
貨期:
編號:B165029
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 M/K-2.7
商品貨號 B165029
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine VCAM-1
Cellular Products
immunoglobulin; monoclonal antibody; against murine VCAM-1
Tumorigenic Yes
Effects
Yes, forms ascites in CB.17.SCID mice
Comments
Animals were immunized with stromal cells from mouse bone marrow.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody blocks adhesion of lymphocyte precursors to bone marrow stromal cells.
The antibody recognizes an antigen believed to be the mouse analog of human VCAM-1 that is expressed on stromal cells in bone marrow and on inflamed endothelial cells (see also M/K-1.9, ATCC CRL-1910).
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 0.5 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1; kappa light chain
Name of Depositor PW Kincade
Deposited As rat (B cell); mouse (myeloma)
References

Miyake K, et al. A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture. J. Cell Biol. 114: 557-565, 1991. PubMed: 1713592

Miyake K, et al. Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. J. Exp. Med. 173: 599-607, 1991. PubMed: 1997648

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
新源县| 松原市| 新民市| 宁阳县| 临猗县| 晋城| 昌乐县| 台山市| 长丰县| 合江县| 伽师县| 丰都县| 南投市| 昌图县| 肇东市| 长沙县| 四会市| 东乡| 兴业县| 景宁| 若羌县| 图木舒克市| 土默特右旗| 迁西县| 阿勒泰市| 修武县| 建湖县| 康乐县| 朔州市| 阿拉善盟| 阿克陶县| 剑河县| 石家庄市| 黎平县| 舟山市| 鄂伦春自治旗| 治县。| 伊吾县| 绍兴县| 福安市| 巴林左旗|