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Jiyoye [Jijoye, P-2003, P-3J]
Jiyoye [Jijoye, P-2003, P-3J]
規(guī)格:
貨期:
編號(hào):B164893
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Jiyoye [Jijoye, P-2003, P-3J]
商品貨號(hào) B164893
Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Burkitt's lymphoma
Age 7 years
Gender male
Ethnicity Black, African
Storage Conditions liquid nitrogen vapor phase
Antigen Expression
HLA A32, B17, Bw37
Genes Expressed
Epstein-Barr virus (EBV)
Cellular Products
Epstein-Barr virus (EBV)
Virus Resistance
The cells are partially resistant to poliovirus and vesicular stomatitis viruses.
Comments
EBNA positive.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 to 6 x 105 viable cells/mL. A maximum of 2 - 3 x 106 viable cells/mL is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
Isoenzymes
G6PD, B
Name of Depositor W Henle
Deposited As Homo sapiens
References

. . J. Clin. Pathol. 38: 261, 1985.

Kohn G, et al. Involvement of C group chromosomes in five Burkitt lymphoma cell lines. J. Natl. Cancer Inst. 38: 209-222, 1967. PubMed: 6019732

. . In Vitro 4: 165, 1969.

Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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