99精品久久99久久久久,亚洲国产成人精品无码一区二区,国产精品亚洲综合色区韩国,久久精品第一国产久精国产宅男66,亚洲国产日韩欧美一区二区三区,久久久久久精品免费无码无,国产中年熟女高潮大集合,亚洲AV无码成H在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Hs 1.Int
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Hs 1.Int
Hs 1.Int
規(guī)格:
貨期:
編號:B164647
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Hs 1.Int
商品貨號 B164647
Organism Homo sapiens, human
Tissue intestine
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age second trimester
Gender male
Ethnicity Caucasian
Karyotype modal number = 46; range = 46 to 55
Clinical Data
male
Caucasian
second trimester
Comments
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Please see the NBL Repository description.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37.0°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
STR Profile
Amelogenin: X Y
CSF1PO: 12, 13
D13S317: 8, 10
D16S539: 9, 11
D5S818: 11, 12
D7S820: 9, 11
THO1: 7
TPOX: 8
vWA: 14, 18
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
修水县| 柏乡县| 平南县| 当涂县| 寿光市| 定日县| 肥乡县| 应城市| 贺兰县| 元江| 长乐市| 开化县| 治县。| 青神县| 虹口区| 邹平县| 玉环县| 鹰潭市| 凤凰县| 谷城县| 大城县| 招远市| 阳谷县| 霞浦县| 金秀| 兴仁县| 虞城县| 城口县| 台东县| 即墨市| 龙口市| 扎鲁特旗| 万州区| 固始县| 西昌市| 民丰县| 大足县| 丹巴县| 九龙县| 抚松县| 通辽市|