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HGF-1
HGF-1
規(guī)格:
貨期:
編號:B164606
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HGF-1
商品貨號 B164606
Organism Homo sapiens, human
Tissue gingival biopsy
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 28 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data
28 years
Caucasian
male
Receptor Expression
bradykinin, expressed
Comments

A longevity study performed by ATCC showed that the depositor's material was capable of growing for an additional 22 population doublings (i.e., unknown + 22) before the onset of senescence. Passage number is different from population doubling level (PDL). Please refer to the Certificate of Analysis for the PDL of the current lot of cells. Current distribution stocks are prepared with only 4-5 Population Doublings (PDLs) remaining under recommended culture conditions after cryopreservation.

Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells.

Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.


Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 11,13
D5S818: 12
D7S820: 10
THO1: 6,7
TPOX: 8,11
vWA: 17,18
Name of Depositor BS McAllister
Deposited As Homo sapiens
Year of Origin October, 1989
References

McAllister BS, et al. Bradykinin receptors and signal transduction pathways in human fibroblasts: integral role for extracellular calcium. Arch. Biochem. Biophys. 304: 294-301, 1993. PubMed: 7686736

McAllister BS, et al. Bradykinin inhibition of EGF- and PDGF-induced DNA synthesis in human fibroblasts. Am. J. Physiol. 265: C477-C484, 1993. PubMed: 8396328

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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