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CC 49 [CC-49, CC49]
CC 49 [CC-49, CC49]
規(guī)格:
貨期:
編號(hào):B164125
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) CC 49 [CC-49, CC49]
商品貨號(hào) B164125
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody binds to TAG-72 with an affinity constant of 20 X 10 exp9 liter/mol.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with affinity purified TAG-72 from LS 174T cells (see ATCC CL-188).
Spleen cells were fused with NS-1 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against TAG-72 human tumor associated glycoprotein
Cellular Products
immunoglobulin; monoclonal antibody; against TAG-72 human tumor associated glycoprotein
Comments
Animals were immunized with affinity purified TAG-72 from LS 174T cells (see ATCC CL-188).
Spleen cells were fused with NS-1 mouse myeloma cells.
The antibody binds to TAG-72 with an affinity constant of 20 X 10 exp9 liter/mol.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; heat-inactivated fetal bovine serum, 15%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype mouse IgG1 kappa
Name of Depositor The United States of America
U.S. Patent Number
References

Schlom J, Colcher D. Second generation monoclonal antibodies having binding specificity to TAG-72 and human carcinomas and methods for employing the same. US Patent 5,512,443 dated Apr 30 1996

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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