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520C9 [520C9.C3B10T]
520C9 [520C9.C3B10T]
規(guī)格:
貨期:
編號:B163777
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 520C9 [520C9.C3B10T]
商品貨號 B163777
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Cancer,Cancer
Applications
By immunofluorescence, the antibody is specific for neoplastic breast tissue with some weak cross-reaction with other neoplasms.
The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human breast cancer cells
Cellular Products
immunoglobulin; monoclonal antibody; against human breast cancer cells
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized either with live cells or with membrane extracts from human breast cancer cell lines.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
By immunofluorescence, the antibody is specific for neoplastic breast tissue with some weak cross-reaction with other neoplasms.
The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium (containing: 0.2 units/mL
insulin, 1 mM Na pyruvate, 0.15 mg/mL oxaloacetic acid), with 4.5 g/L glucose,
60%; NCTC 109 medium, 20%; fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Cetus Corp.
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Ring DB, et al. Monoclonal anti-human breast cancer antibodies. US Patent 4,753,894 dated Jun 28 1988

Tempest PR, et al. Humanized antibodies to Fc receptors for immunoglobulin G on human mononuclear phagocytes. US Patent 6,500,931 dated Dec 31 2002

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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