99精品久久99久久久久,亚洲国产成人精品无码一区二区,国产精品亚洲综合色区韩国,久久精品第一国产久精国产宅男66,亚洲国产日韩欧美一区二区三区,久久久久久精品免费无码无,国产中年熟女高潮大集合,亚洲AV无码成H在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 1B2A3
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
1B2A3
1B2A3
規(guī)格:
貨期:
編號:B163615
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 1B2A3
商品貨號 B163615
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody differentiates toxin producing strains of P. multocida from other isolates, and can be used to identify toxigenic strains of P. multocida.
The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with a toxoid preparation purified from the NOR-1 strain of P. multocida (the toxoid preparation was a gift from J.C. Frantz).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990.
Genes Expressed
immunoglobulin; monoclonal antibody; against Pasturella multocida type D dermonecrotic toxin
Cellular Products
immunoglobulin; monoclonal antibody; against Pasturella multocida type D dermonecrotic toxin
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with a toxoid preparation purified from the NOR-1 strain of P. multocida (the toxoid preparation was a gift from J.C. Frantz).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody differentiates toxin producing strains of P. multocida from other isolates, and can be used to identify toxigenic strains of P. multocida.
The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor LA Elsken
Originators: KW Ruby,W Knudtson
Deposited As mouse (B cell); mouse (myeloma)
Year of Origin 1990
References

Martineau-Doize B, et al. Effects of purified Pasteurella multocida dermonecrotoxin on cartilage and bone of the nasal ventral conchae of the piglet. Anat. Rec. 228: 237-246, 1990. PubMed: 2260779

Magyar T, Rimler RB. Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay. J. Clin. Microbiol. 29: 1328-1332, 1991. PubMed: 1885729

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
平乡县| 家居| 荆门市| 宣城市| 宜黄县| 永定县| 靖宇县| 拜泉县| 吉木萨尔县| 尚志市| 扎囊县| 明光市| 德江县| 东台市| 怀安县| 旬阳县| 达尔| 志丹县| 鄂尔多斯市| 成都市| 雷山县| 红桥区| 海城市| 会同县| 榆社县| 当雄县| 自治县| 巨鹿县| 曲沃县| 新昌县| 鸡泽县| 陆良县| 香港 | 通辽市| 上犹县| 睢宁县| 驻马店市| 嵩明县| 延寿县| 大洼县| 集贤县|