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Animals were immunized with a synthetic peptide with the sequence LGSGAFGTIYKG(C) corresponding to residues 138-149 of the v-erb B oncogene.

The 170 kd EGF receptor is weakly detected in immunoblot screening of A431 cells.

In addition, a protein of approximately 100 kd is detected in extracts of human brain.

Spleen cells were fused with Sp2/0 myeloma cells.

immunoglobulin; monoclonal antibody; against a synthetic v-erb B oncogene peptide

immunoglobulin; monoclonal antibody; against a synthetic v-erb B oncogene peptide

Animals were immunized with a synthetic peptide with the sequence LGSGAFGTIYKG(C) corresponding to residues 138-149 of the v-erb B oncogene.

Spleen cells were fused with Sp2/0 myeloma cells.

In ELISA tests using synthetic peptides representing erb B, fes/fps, fgr, fms, src/yes, mil/raf, abl, mos, CDC28 and the catalytic and regulatory subunit of cyclic AMP dependent protein kinase, the antibody recognizes only the erb B sequence.

The 170 kd EGF receptor is weakly detected in immunoblot screening of A431 cells. In addition, a protein of approximately 100 kd is detected in extracts of human brain.

The IgG1 and IgG2b reactivities of the antibody with the v-erb B peptide (138 to 149) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

culture medium 95%; DMSO, 5%

Temperature: 37.0°C