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From tumor that arose in a mouse that had been inoculated with +/+ Li cells (ATCC CRL-6467, embryonic liver).

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

culture medium 95%; DMSO, 5%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C