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MEG-01
MEG-01
規(guī)格:
貨期:
編號:B161440
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MEG-01
商品貨號 B161440
Organism Homo sapiens, human
Tissue bone marrow
Cell Type megakaryoblast
Product Format frozen
Morphology lymphoblast
Culture Properties mixed, adherent and suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease chronic myelogenous leukemia (CML)
Age 55 years
Gender male
Applications This cell line is a suitable transfection host.
Karyotype hyperdiploid; modal number = 56 to 58; the Philadelphia chromosome (Ph1) is present
Images
Derivation
The MEG-01 cell line was derived in 1983 at the Nagoya University School of Medicine, Nagoya, Japan from bone marrow cells taken from a patient in megakaryoblastic crisis of CML.
Clinical Data
male
55 years
Antigen Expression
CD41 +; CD61 +; CDw14 +
Comments
The cells are positive for cytoplasmic Factor VIII and surface GPIIb/IIIa, periodic acid - Schiff (PAS) reaction, alpha naphthyl acetate esterase and acid phosphatase.
They are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase and alkaline phosphatase.
The stain positively with monoclonal antibodies BA-1 (anti B cell, granulocyte), HPL-3 (anti gpIIb/IIIa) and 20.3 (anti monocyte, platelet).
They are negative for other lymphoid and myeloid seris antigens.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Subcultures are prepared by scraping the adherent cells into the medium. From the resulting suspension dilute cells to a concentration 1-2 X 105 cells/mL into fresh medium in a new flasks. Keep culture below approximate density of 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping the adherent cells into the medium, and diluting the resulting suspension into fresh medium in new flasks.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 8
D16S539: 9
D5S818: 13
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16
Population Doubling Time 36 to 48 hrs
Name of Depositor M Tekeuchi, MJ Fitzgerald
Deposited As Homo sapiens
Year of Origin 1983
References

Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511

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